Fig. 7.
![Fig. 7. Fibrin clot retraction mediated by the cells stably expressing mutant αIIbβ3. / 293 cells (control), 293 cells stably expressing wild-type β3 alone, wild-type αIIbβ3, Tyr143HisαIIbβ3, or KO mutant αIIbβ3 suspended in Tyrode/HEPES buffer (2 × 106 cells/mL) were incubated with 10 mM tranexamic acid, 250 μg/mL fibrinogen, and 2 mM CaCl2 at 37°C in a siliconized glass tube in the presence of 50 μM cyclo(RGDfV) (αvβ3-specific antagonist). Then 1 U thrombin was added to 1 mL cell suspension. Clot retraction was monitored by digital photography every 30 minutes. Data were expressed as % clot retraction = [(area t0 − areat)/area t0] × 100. Results are representative of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/9/10.1182_blood-2002-07-2144/4/h80934230007.jpeg?Expires=1722613333&Signature=Ojr7OHRlj1WzkihM3gJnlBjvhicqzahsP-pHpTWC-UVdfewrEpNWdYPB5GMaO~dfXtlkGQ2PalB-9CAVIYQWzWNQiOKpFvDPz9IAgkhjY01nP1XdEnKh3caJTyRmJIO2fPCJZQj5eeCPF2R8J8MAVtmFrJF3bLzzE1srK222eKxM-urblckbfcSDTMHfm8jI2Y1DYilx4J0~CmBcoqE6OK~Gfjz2u4psSVcnkvBEuV2lqY74rYDGIp5jfQ3yCEbz~4wlE86d~q~T5a58pCGG2bRa-UR-cVQ97IP5XwGD5wu6SnHK~H7pGN6kjBzkYY-cX5itTQLSwnyx7ARpbqLjbQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fibrin clot retraction mediated by the cells stably expressing mutant αIIbβ3.
293 cells (control), 293 cells stably expressing wild-type β3 alone, wild-type αIIbβ3, Tyr143HisαIIbβ3, or KO mutant αIIbβ3 suspended in Tyrode/HEPES buffer (2 × 106 cells/mL) were incubated with 10 mM tranexamic acid, 250 μg/mL fibrinogen, and 2 mM CaCl2 at 37°C in a siliconized glass tube in the presence of 50 μM cyclo(RGDfV) (αvβ3-specific antagonist). Then 1 U thrombin was added to 1 mL cell suspension. Clot retraction was monitored by digital photography every 30 minutes. Data were expressed as % clot retraction = [(area t0 − areat)/area t0] × 100. Results are representative of 3 separate experiments.
