Morphologic analysis of the adherent cells stably expressing mutant α_IIbβ₃ to immobilized fibrinogen.

293 cells stably expressing (A) wild-type α_IIbβ₃, (B) Tyr143Hisα_IIbβ₃, or (C) KO mutant α_IIbβ₃ were incubated with immobilized fibrinogen (5 μg/mL) at 37°C for 60 minutes in the presence of 50 μM cyclo(RGDfV) (α_vβ₃-specific antagonist). After gentle washing with PBS, adherent cells were fixed in 3.7% formaldehyde and permeabilized in 0.5% Triton X-100. Cells were stained with rhodamine–phalloidin and analyzed under a fluorescence microscope. Original magnification, × 100.