Fig. 1.
Flow cytometric analysis of the surface expression and ligand-binding function of αIIbβ3 Osaka-12 platelets.

Flow cytometric analysis of the surface expression and ligand-binding function of αIIbβ3 Osaka-12 platelets.

Washed platelets obtained from Osaka-12 or 3 control subjects were incubated with FITC-AP1 (GPIb-specific mAb), FITC-AP2 (αIIbβ3-specific mAb), FITC-AP3 (β3-specific mAb), and FITC-TP80 (αIIb-specific mAb) at a concentration of 10 μg/mL for 30 minutes at room temperature. FITC-MOPC21 (mouse myeloma IgG1) was used as a negative control. For OP-G2 binding (activation-independent ligand-mimetic αIIbβ3-specific mAb), bound antibodies were detected by FITC-conjugated goat F(ab′)2 antimouse IgG. Filled and open histograms represent the binding of specific and control antibodies, respectively. For PAC-1 binding, washed platelets were preincubated with 10 μg/mL PT25-2 (activating αIIbβ3-specific mAb) for 30 minutes, and then 10 μg/mL FITC-labeled PAC-1 was added. Closed and open histograms represent the PAC-1 binding in the absence and presence of 10 μM FK633 (αIIbβ3-specific antagonist), respectively. Results are representative of 2 separate experiments.

Close Modal

or Create an Account

Close Modal
Close Modal