PF-4 has no effect on Akt phosphorylation.

Serum-deprived ACE were stimulated for 10 minutes with FGF2 (10 ng/mL) and various concentrations of PF-4 (1 μg/mL to 10 μg/mL). Cell lysates were analyzed by Western blotting, using polyclonal antibodies against phosphorylated Akt or total Akt. Results are representative of 3 experiments.