![Fig. 5. Additive effects of PI3K and ERK pathways on ACE cell proliferation. / (A) Effect of PD98059 and Ly294002 on FGF2-induced DNA biosynthesis. Serum-deprived ACE cells were stimulated with FGF2 (10 ng/mL) in the presence of various concentrations of Ly294002 (1 μM to 10 μM) with (dotted line) or without (plain line) PD98059 (5 μM). DNA biosynthesis was determined by [3H]TdR incorporation during a 20-hour pulse, as described in “Materials and methods.” Data are expressed as means of triplicate values ± SEM and are representative of 6 experiments. (B) Effect of PD98059 and Ly294002 on ERK and Akt phosphorylation. Serum-deprived ACE cells were stimulated with FGF2 (10 ng/mL) in the presence of various concentrations of PD98059 (2 μM to 20 μM) or Ly294002 (1 μM to 10 μM). Cell lysates were analyzed by Western blotting, using polyclonal antibodies recognizing ERK-P and total ERK, and phosphorylated or total Akt. Results are representative of 4 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/9/10.1182_blood.v100.9.3087/4/h82123347005.jpeg?Expires=1724156667&Signature=IpXA2kGhUmxn0~LETwN162x5ZRXscq4a0wb9-NP~WRIIi3596LHuYpjrbQbZhCzxT9gfDQFHJYhq5wYY0XrIOMur3mIZ9kXyzS8B-ExDTGGY5Pr16oUNFnisPN4CCwVQPv0aO~KAu49I0GQ85CkEwnSdbrUTtETf~2qxNKR0cJHxFFLImpxfi8vmfMBYeicUtuDjQZ7YOoxF98yfwL2XGys-hnaZ4NW-Ck5UI8Jcf0caCgaln3StuYiDGWqxt-JXukZjLUiNUcJyoVuR27Rydwj2yMjaOZMhIq~ojtk2hjbbQrUKcqIOqxw30nAOW-wDmNTIJSS4rL3gpVXJG3oQMw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Additive effects of PI3K and ERK pathways on ACE cell proliferation.
(A) Effect of PD98059 and Ly294002 on FGF2-induced DNA biosynthesis. Serum-deprived ACE cells were stimulated with FGF2 (10 ng/mL) in the presence of various concentrations of Ly294002 (1 μM to 10 μM) with (dotted line) or without (plain line) PD98059 (5 μM). DNA biosynthesis was determined by [3H]TdR incorporation during a 20-hour pulse, as described in “Materials and methods.” Data are expressed as means of triplicate values ± SEM and are representative of 6 experiments. (B) Effect of PD98059 and Ly294002 on ERK and Akt phosphorylation. Serum-deprived ACE cells were stimulated with FGF2 (10 ng/mL) in the presence of various concentrations of PD98059 (2 μM to 20 μM) or Ly294002 (1 μM to 10 μM). Cell lysates were analyzed by Western blotting, using polyclonal antibodies recognizing ERK-P and total ERK, and phosphorylated or total Akt. Results are representative of 4 experiments.
