Fig. 1.
![Fig. 1. PF-4 inhibits both basal and FGF2-induced DNA biosynthesis in ACE cells. / (A) Serum-deprived ACE cells were cultured with (black bars) or without (white bars) FGF2 (10 ng/mL) in the presence of various concentrations of PF-4 (5 μg/mL and 10 μg/mL). (B) Serum-deprived ACE cells were cultured without FGF2 in the presence of anti-FGF2 and anti–FGF-R1 antibodies. DNA biosynthesis was determined by [3H]TdR incorporation into DNA during 20 hours of incubation, as described in “Materials and methods.” Data are expressed as a percentage of incorporation into control cells (A: 32 650 cpm/well = 100%), (B: 32 183 cpm/well = 100%). Data are expressed as the means ± SEM of 3 independent experiments, each performed in triplicate.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/9/10.1182_blood.v100.9.3087/4/h82123347001.jpeg?Expires=1724157579&Signature=hrjL5g-n~Hn3bO9eLQTcGzfYFgYUlYwoLS6GgDSM33WAehEGJKZcwLQk5rHsaqua1C4yxUqSFzrBQ46dXbHKFUQsIQK82tTVyTBVjFliSre0QXkiArVoQw68Jq~o3jIW3RWkY9kY9XgVmGpsJbSNgbNGsvRF0eqWDdx5TfIJMdeEYZauHX4-LnL6WiUiAHDXx4442SHddK68iwyB3Vcw-BTorutAoMgqgAVfoM5ryJHWTTHS9LPD-Hz5r1qndbherJ-jLEFiLL8j-j1nKVi~UtGH~Js5qaKm5sIzl9siCNOG1j90TbfoOaSbYnMRf2l0G4zoguKYZMUXdO~w6Tjrnw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PF-4 inhibits both basal and FGF2-induced DNA biosynthesis in ACE cells.
(A) Serum-deprived ACE cells were cultured with (black bars) or without (white bars) FGF2 (10 ng/mL) in the presence of various concentrations of PF-4 (5 μg/mL and 10 μg/mL). (B) Serum-deprived ACE cells were cultured without FGF2 in the presence of anti-FGF2 and anti–FGF-R1 antibodies. DNA biosynthesis was determined by [3H]TdR incorporation into DNA during 20 hours of incubation, as described in “Materials and methods.” Data are expressed as a percentage of incorporation into control cells (A: 32 650 cpm/well = 100%), (B: 32 183 cpm/well = 100%). Data are expressed as the means ± SEM of 3 independent experiments, each performed in triplicate.
