Fig. 7.
Fig. 7. A Stat5-binding site is present in the RARE within the RARβ promoter. / (A) Sequence of the RARE within the RARβ promoter (−55 to −33).21 The two 6-bp direct repeats making up the DR5 element are in bold. (B) Nuclear extracts from BaF3 cells transfected with a Stat5 expression vector were used in an EMSA or an EMSA supershift with the indicated labeled probes and antibody. The Stat5 oligo harbors a consensus Stat5-binding sequence (see “Materials and methods” for this sequence). (C) The βRAREm4 oligo, which harbors mutations within the 6-bp direct repeats while retaining the consensus Stat-binding site (see “Materials and methods” for the exact sequence) served as a probe in EMSAs using BaF3 nuclear extracts and different Stat antibodies as indicated. (D) The labeled Stat5 oligo served as a probe in EMSAs using BaF3 nuclear extracts. Competition reactions were performed with the addition of an excess of different cold oligos as indicated.

A Stat5-binding site is present in the RARE within the RARβ promoter.

(A) Sequence of the RARE within the RARβ promoter (−55 to −33).21 The two 6-bp direct repeats making up the DR5 element are in bold. (B) Nuclear extracts from BaF3 cells transfected with a Stat5 expression vector were used in an EMSA or an EMSA supershift with the indicated labeled probes and antibody. The Stat5 oligo harbors a consensus Stat5-binding sequence (see “Materials and methods” for this sequence). (C) The βRAREm4 oligo, which harbors mutations within the 6-bp direct repeats while retaining the consensus Stat-binding site (see “Materials and methods” for the exact sequence) served as a probe in EMSAs using BaF3 nuclear extracts and different Stat antibodies as indicated. (D) The labeled Stat5 oligo served as a probe in EMSAs using BaF3 nuclear extracts. Competition reactions were performed with the addition of an excess of different cold oligos as indicated.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal