Light cell generation from dense cells.

Dense cells from fraction 5 (F5, ρ > 1.107 g/mL) of the primary gradient were isolated. A second, identical gradient was performed, and the percentage of total SSRBCs in each of the 5 density fractions was determined in buffer A for the following conditions: (1) immediately after the primary density gradient (n = 4); (2) after 90 minutes of 37°C oxy incubation (n = 42); and (3) after oxy/deoxy cycling (n = 42). Density ranges for each fraction were as follows: F1: ρ < 1.077; F2: 1.077 < ρ < 1.087; F3: 1.087 < ρ < 1.097; F4: 1.097 < ρ < 1.107; F5: ρ > 1.107.