Fig. 7.
IL-10 protects B cells from cell death.
(Top) Bulk B cells were stimulated with F(ab′)2 anti-IgM (10 μg/mL) and CD40 ligand trimer with or without neutralizing anti–IL-10 antibodies. Cells were then stained at day 7 with FITC-labeled Annexin V and PI and were analyzed using fluorescence-activated cell sorting (FACS). (Bottom) Purified CD5− and CD5+ B cells were activated as above for 48 hours, and apoptosis was measured at day 4. Results are representative of 3 experiments.
