Fig. 6.
Fig. 6. CD5 constitutively activates human IL-10 promoter. / Daudi-LZRS or Daudi-LZRS.CD5 cells stimulated or not stimulated with 2 μg/mL F(ab′)2 anti-IgM were cotransfected withRenilla Luciferase control reporter vector pRL-SV40 and each of the different pGL2 vectors, pGL2-Basic and pGL2-1308 (containing the human IL-10 promoter/enhancer region). To take into account different transfection efficiencies, firefly luciferase activity of the pGL2 vectors was normalized to Renilla luciferase activity within each experimental setting and displayed as arbitrary units. Four different electroporation assays for each condition were conducted. Shown are the control activity of pGL2 basic vector and the basal activity (unstimulated) and anti-IgM–induced activity of pGL2-1308 vector (stimulated) in CD5− (dotted) and CD5+cells (gray). Data shown are means ± SD of 4 experiments.

CD5 constitutively activates human IL-10 promoter.

Daudi-LZRS or Daudi-LZRS.CD5 cells stimulated or not stimulated with 2 μg/mL F(ab′)2 anti-IgM were cotransfected withRenilla Luciferase control reporter vector pRL-SV40 and each of the different pGL2 vectors, pGL2-Basic and pGL2-1308 (containing the human IL-10 promoter/enhancer region). To take into account different transfection efficiencies, firefly luciferase activity of the pGL2 vectors was normalized to Renilla luciferase activity within each experimental setting and displayed as arbitrary units. Four different electroporation assays for each condition were conducted. Shown are the control activity of pGL2 basic vector and the basal activity (unstimulated) and anti-IgM–induced activity of pGL2-1308 vector (stimulated) in CD5− (dotted) and CD5+cells (gray). Data shown are means ± SD of 4 experiments.

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