CD5+ B cells produce more IL-10 than CD5− B cells following BCR stimulation.
(A) Kinetic analysis of IL-10 production. Purified B cells were cultured in medium alone or with F(ab′)2 anti-IgM (10 μg/mL) and CD40 ligand trimer. Supernatants of 105 cells were harvested sequentially until day 7, and IL-10 was measured using ELISA. Shown are the means from 2 experiments. (B) Respective IL-10 production by CD5− and CD5+ activated blood B cells. CD5− ST and CD5+ ST 48 hours. Negatively selected B cells were stained with anti-CD3 and anti-CD5 mAbs and were sorted based on CD3−CD5− and CD3−CD5+ expression. Thereafter 105 cells were cultured in medium with F(ab′)2anti-IgM (10 μg/mL) and CD40 ligand trimer for 48 hours, and supernatants were harvested for IL-10 production assay.
