American Society of Hematology

Fig. 1.

$Fig. 1. CD5+ peripheral blood B cells do not belong to an activated subset, and only a fraction of activated B cells express CD5 following activation. / B cells were purified by negative selection on beads from normal PBMCs leading to a greater than 90% pure population. Cells were double stained with mAbs, before (top and middle) or after activation with F(ab′)2 anti-IgM (10 μg/mL) and CD40 ligand trimer (2.5 μg/mL final) for 48 hours (bottom). Percentages of positive cells are shown on bars or inside quadrants.$

CD5⁺ peripheral blood B cells do not belong to an activated subset, and only a fraction of activated B cells express CD5 following activation.

B cells were purified by negative selection on beads from normal PBMCs leading to a greater than 90% pure population. Cells were double stained with mAbs, before (top and middle) or after activation with F(ab′)₂ anti-IgM (10 μg/mL) and CD40 ligand trimer (2.5 μg/mL final) for 48 hours (bottom). Percentages of positive cells are shown on bars or inside quadrants.

This Feature Is Available To Subscribers Only