Equivalent efficiency of influenza virus–infected pDCs and CD11c⁺ DCs to activate influenza A virus Aichi/68 memory CD4⁺ T-cell response.

T cells were cocultured with pDCs or CD11c⁺ DCs infected by influenza virus. After 7 days, T cells were restimulated by autologous monocyte-derived mature DCs infected or not with influenza virus in the presence of brefeldin-A. Production of IFN-γ by CD4⁺ T cells was assessed by intracytoplasmic staining and flow cytometry.