Influence of antigen forms on influenza antigen HLA class I and II presentation by pDCs and CD11c⁺ DCs.

(A) Flu16, an HLA-A*0201–restricted influenza MP_(58-66)-specific CD8⁺ T-cell clone, was cocultured with HLA-A*0201⁺ pDCs or CD11c⁺ DCs previously exposed to live, heat-inactivated (HI) or boiled influenza virus. After 3 days, ³H-thymidine was added for 12 hours to measure proliferation. (B) HA136, a DRα1*0101/DRβ*0401-restricted influenza HA_(307-319)–specific CD4⁺ T-cell clone, was cocultured with DRα1*0101/DRβ*0401⁺ pDCs or CD11c⁺ DCs previously exposed to live, heat-inactivated or boiled influenza virus. After 3 days,³H-thymidine was added for 12 hours to measure proliferation.