Gross and microscopic appearance of NXS2-I-GFP and NXS2-TIMP-3-I-GFP-(high) tumors.

Gross appearance after reflection of the overlying skin and exposure of the subcutaneous tumors (A,B; marks are 1 cm apart). Immunohistochemical analysis of intratumoral vascularity as assessed by staining with anti–CD31 antibody (C,D; original magnification, × 25); intratumoral vessel SMA content (E,F; original magnification, × 25); intratumoral VE-cadherin expression (G,H; original magnification, × 25); and confocal microscopic analysis of functional vascular status demonstrated by the inherent autofluorescence of intravascular erythrocytes (I,J). NXS2-I-GFP tumors, left panels; NXS2-TIMP-3-GFP-(high) tumors, right panels.