Effect of conditioned medium from NXS2 tumor cells overexpressing TIMP-3 on VEGF-stimulated HUVEC migration and bFGF-stimulated tubule formation.

(A) Data (means ± SE of triplicates) are expressed as numbers of migrating cells across the filter area. Also shown are the dose-dependent effects of diluting TIMP-3 at ratios of 1:2 and 2:1, the effect of medium with and without VEGF, and the effect of 250 ng/mL recombinant TIMP-3. (B) Tubule formation in the presence of NXS2-I-GFP–conditioned medium (CM). (C) Inhibition of tubule formation in the presence of NXS2-Timp-3-I-GFP CM.