Radiosensitivity of AT fibroblast cell transfectants.

(A) Radiosensitivity of GM05849C cells transfected with pcDNA3-YZ5 and pcDNA3-YZ5/8921T was determined by clonogenic assay. Cells were exposed to 0, 1, 2, 3, 4, or 5 Gy X-IR and then cultured for 10 days prior to fixation, staining, and assessment of colony formation. The assay was performed in triplicate; mean colony numbers relative to unirradiated controls were plotted and the SE was shown as an error bar.(B) Expression of ATM protein was equivalent between GM05849C cells transfected with pcDNA3-YZ5 and pcDNA3-YZ5/8921T. Western blot analysis was performed with anti-ATM antibody. α-Tubulin was used as a control for protein loading.