![Fig. 1. Cytotoxicity and intracellular Ca++ release of 4D4 CTL clone are inhibited by the CD158a receptor. / (A) Cytotoxicity of CD158a+ CTL clone 4D4 was assessed at a 3:1 E/T ratio against RCC and EBV-B–transformed cell lines (LCL) derived from 3 patients: autologous RCC-7 (HLA-A2, Cw4), allogeneic RCC-6 (HLA-A2, Cw3), and RCC-5 (HLA-A9, Cw3). Cytotoxicity was determined in absence (medium) or presence of anti–HLA-B/C or control anti–HLA-DR mAbs (1 of 5 experiments is shown). Error bars indicate standard deviation. (B) Flow cytometry analysis of [Ca++]i release by Fluo3-am–loaded CTL stimulated for 1 minute at 37°C with PKH26-labeled target cells. Percentages correspond to CTL conjugates with calcium release of total CTLs. Target cell profile of LCL-7 alone is represented (left panel). (C) [Ca++]imeasurements were calculated from mean fluorescence values (see “Materials and methods”). Determination of [Ca++]i release by CTLs stimulated by RCC cells (left panel), LCL-6 or LCL-7, coated or not with mAbs (right panel).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/8/10.1182_blood-2002-02-0643/4/h82023240001.jpeg?Expires=1725456717&Signature=KKZCpKCnWxGGe718knLgOnGfYnmukKINSkzc-TZn5-wm~0TkCI3fiM78hAncX03CSVx4XPHNZUVASg-VBwliW8iN8saSNy09NHwqHljQVgCdilG383AXB9w-oYwNkmxmUobq~GFImN72y8VUsnTOSCiNOzGNqch-54W7XOJgzsKQw8ZNSk8i7EqUbEu-3JeqYTEvYtOqP1eCYlbNY82wXFPIAIDEKTtSm2crwqCn7d4PIXtU5uEBgoLNqvQEnplX-a64ZcGRNQ25P7nuxXeKtBcwH-TX9oRn4QLTZ23oOIpzcZdp4i2o6nLvBUKAHA2Tt9W0D6pKo5fPwwx6xW8SgA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cytotoxicity and intracellular Ca++ release of 4D4 CTL clone are inhibited by the CD158a receptor.
(A) Cytotoxicity of CD158a+ CTL clone 4D4 was assessed at a 3:1 E/T ratio against RCC and EBV-B–transformed cell lines (LCL) derived from 3 patients: autologous RCC-7 (HLA-A2, Cw4), allogeneic RCC-6 (HLA-A2, Cw3), and RCC-5 (HLA-A9, Cw3). Cytotoxicity was determined in absence (medium) or presence of anti–HLA-B/C or control anti–HLA-DR mAbs (1 of 5 experiments is shown). Error bars indicate standard deviation. (B) Flow cytometry analysis of [Ca++]i release by Fluo3-am–loaded CTL stimulated for 1 minute at 37°C with PKH26-labeled target cells. Percentages correspond to CTL conjugates with calcium release of total CTLs. Target cell profile of LCL-7 alone is represented (left panel). (C) [Ca++]imeasurements were calculated from mean fluorescence values (see “Materials and methods”). Determination of [Ca++]i release by CTLs stimulated by RCC cells (left panel), LCL-6 or LCL-7, coated or not with mAbs (right panel).
