![Fig. 1. PML binds to STAT3 and inhibits its activity. / NIH3T3 (A,F), 293T (B), HepG2 (C,E,G), and 32D (D) cells were transfected with an appropriate reporter gene (4 × APRE-Luc [A-D], 4 × ISRE-Luc [E], or 3 × β-Cas-Luc [F-G]) along with the indicated expression vectors. Also, 293T cells were transfected with pEF-BOS-G-CSF-R/gp130 and HepG2 cells with G-CSF-R. Relative luciferase activities induced by IL-6, G-CSF, EPO, IFNα, or 1*6-STAT5 were quantitated. The results are shown as the means ± standard deviations (SDs) of triplicate cultures. Similar results were obtained from at least 4 independent experiments. (H-I) 293T cells were transfected with pEF-BOS-G-CSF-R/gp130 and the indicated expression vectors. After G-CSF stimulation, total cell lysates were isolated and subjected to immunoprecipitation (IP) and immunoblotting (IB) with the indicated antibodies.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/9/10.1182_blood-2002-08-2474/4/h80934218001.jpeg?Expires=1723400672&Signature=oKMHzYLM5tWWFRekvNRAyikqHIbV9-dV5SFR6lMw2~9FW8OQ-BmOsKK61XZNYRvz23GvlUhFghrx5AqGLSy4PGd9hTMafWGbWuZKJC868eWSkWWRwZ8GWH7oAOYOnj53g1e6JE3HAOc4UYXB1eMwFQeDwKRgtJtQR68Vr0c~ZKQNZODeQMn283BkcUSZRJTEkEH9CA7o2v~a0R~VA1~rIanrIOK3zw0qJ-5qJCFoBP5yui9~PUGefll~X0gmThE8zSNi8SQUdgkveLIJw5c2caRC8vBEhImENCrwSWoRunYd89KNWv-wW9zRRS0tcX1eOcHomrqnfmMPASvZKBR5gg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PML binds to STAT3 and inhibits its activity.
NIH3T3 (A,F), 293T (B), HepG2 (C,E,G), and 32D (D) cells were transfected with an appropriate reporter gene (4 × APRE-Luc [A-D], 4 × ISRE-Luc [E], or 3 × β-Cas-Luc [F-G]) along with the indicated expression vectors. Also, 293T cells were transfected with pEF-BOS-G-CSF-R/gp130 and HepG2 cells with G-CSF-R. Relative luciferase activities induced by IL-6, G-CSF, EPO, IFNα, or 1*6-STAT5 were quantitated. The results are shown as the means ± standard deviations (SDs) of triplicate cultures. Similar results were obtained from at least 4 independent experiments. (H-I) 293T cells were transfected with pEF-BOS-G-CSF-R/gp130 and the indicated expression vectors. After G-CSF stimulation, total cell lysates were isolated and subjected to immunoprecipitation (IP) and immunoblotting (IB) with the indicated antibodies.
