MS-5–induced proliferation and differentiation of CD34⁺ cells.

CD34⁺ cells were plated at 1 × 10⁴ cells/mL on MS-5 feeder cells with or without the addition of 20 μg/mL NU-L_PAN. After 2 weeks, the number of cells was determined (A). NU-L_PAN prohibited erythroid maturation as demonstrated by Wright staining (B); original magnification, × 125. CD34⁺ cells and cultured cells were subjected to colony formation assay and the numbers of each CFU-GEMM, CFU-GM, and BFU-E were determined (C). For panels A and C, data are represented as the means ± SD (n = 3).