Forced α1-3-fucosylation of WEHI-3 cells or murine neutrophils with FTVI and GDP-fucose creates many sLe^x and Le^x epitopes but does not significantly increase selectin ligands.

WEHI-3 cells or murine neutrophils were incubated with or without FTVI and GDP-fucose. Some of the FTVI-treated WEHI-3 cells were subsequently treated with sialidase. The cells were analyzed for binding of control IgM or the anti-sLe^x mAb HECA-452 or for binding of control CD45 IgM chimera or P- or E-selectin IgM chimera. The data are representative of at least 8 experiments.