RT-PCR reveals mRNA for FTVII and FTIV in WEHI-3 cells.

Total RNA from murine spleen and lung, used as positive controls, and human HL-60 cells, used as a negative control, and from WEHI-3 cells were incubated in the presence or absence of reverse transcriptase. The samples were then amplified by PCR using primers for murine FTVII or FTIV. The reaction products were resolved on an agarose gel and stained with ethidium bromide. The data are representative of 5 experiments.