Most CD3 CD8 T cells specifically producing IFN-γ in response to proteinase 3 peptide are Granzyme B⁺CD45RA⁺ CCR7⁻ CXCR4⁻.

Analysis of PBMCs was performed by 4-color flow cytometry in patient 10. (A, B) Granzyme B (Gra B)/IFN-γ profile of CD3/CD8-gated lymphocytes. (C, D) CD45RA/IFN-γ profile of CD3/CD8-gated lymphocytes. (E, F) CCR7/IFN-γ profile of CD3/CD8-gated lymphocytes. (G, H) CXCR4/IFN-γ profile of CD3/CD8-gated lymphocytes. Frequencies of CD3 CD8 T cells that produced IFN-γ are shown in each quadrant as a percentage of the total number of CD3 CD8 T cells.