Flow cytometric analysis of T-cell responses to WT1 126-134, WT1 187-195, and proteinase 3 in AML patients.

Analysis of PBMCs was performed by 3-color flow cytometry. Figures show the CD8/IFN-γ profile of CD3-gated lymphocytes in unstimulated (left) and peptide-exposed (right) PBMCs in the 7 patients with specific T-cell responses to the indicated peptides. The T-cell response in patient 1 was analyzed against both WT1 peptides in one sample. Frequencies of CD3 CD8 T cells that produced IFN-γ are shown as percentages of the total number of CD3 CD8 T cells. APC indicates allophycocyanin; FITC, fluorescein isothiocyanate.