MIP-1α induces AKT phosphorylation in MM cells.

(A) MM1.S cells and (B) pat MM cells were incubated with MIP-1α (100 ng/mL) for 15 minutes. Pretreatment with PI3-K inhibitor wortmannin (1 μM) or LY 294002 (50 μM) was performed for 1 hour prior to cytokine addition. Pretreatment with either wortmannin or LY 294002 yielded similar results in MM1.S and pat MM cells (data not shown). Cells were lysed, electrophoresed, and immunoblotted with antiphospho-AKT (Ser473) antibody and AKT antibody as loading control (ctr).