Quantitative real-time PCR analysis of BM from a tertiary NOD/SCID mouse .

GFP transgene amplification curves, obtained by real-time quantitative PCR analysis of 100 ng of DNA from the BM of tertiary mice (Ct: 30.1).The analysis was performed as described for Figure 4C and indicates that BM of this mouse contained 0.004 copies of vector per total genome analyzed (human plus murine). The corresponding FACS analysis (0.43% GFP⁺ cells on total BM cells) is represented in Table 3 (mouse A2.1.1). The Southern blot analysis of this particular tertiary mouse is also represented in Figure 5B. One of 2 analyses giving similar results is shown. NT indicates untreated mouse.