Cytotoxic effect of TRAIL against Ph1-positive leukemia cell lines.
(A) Dose response of growth inhibition. CML-BC–derived (left panel) or Ph1-positive AL-derived (right panel) cell lines were cultured for 42 hours in the absence or presence of the indicated concentrations of rhsTRAIL, and the 3H-thymidine uptake was evaluated for the last 6 hours. T-cell leukemia cell lines (Jurkat and MOLT4F) were also included as controls and are shown by the dotted lines. Data with myeloid cell lines are shown as the closed symbols. All data are represented as the mean of triplicate samples. Standard errors were always less than 10% (not shown). (B) Neutralization by anti-TRAIL mAb. Nalm1 cells were cultured for 42 hours with rhsTRAIL (10 ng/mL) in the presence or absence of neutralizing anti-TRAIL mAb (10 μg/mL), and the 3H-thymidine uptake was evaluated for the last 6 hours. Data are represented as the mean ± SD of triplicate samples. (C) Time course of cell viability. CML-BC–derived (left panel) or Ph1-positive AL-derived (right panel) cell lines were cultured with 100 ng/mL of rhsTRAIL for 12, 24, or 36 hours, and then the viability was determined by the trypan blue exclusion assay. Data with Jurkat and MOLT4F are also shown by the dotted lines. Data with myeloid cell lines are shown as the closed symbols. All data are represented as the mean of triplicate samples. Standard errors were always less than 10% (not shown).
