Methods of avoiding CAR T-cell escape (A-C) or limiting CAR T-cell targeting to allow use of multiple antigens for either cell specificity or avoidance (D-E): targeting 2 antigens with the same construct (A), targeting 2 antigens with separate constructs in a single cell (B), targeting antigens with pools of cells containing the 1 or the other construct (C), positive gating strategy (2 antigens on the tumor required for killing) (D), and negative gating strategy (a second antigen present on a normal cell shuts down killing) (E). Illustration by Patrick Lane, ScEYEnce Studios.