Fig. 5.
Dependency of specific inhibition by RhD peptides on IL-10 and CTLA-4.
RhD peptides that elicit IL-10 responses were used to inhibit proliferation by cultures of PBMCs from AIHA patient 1 that had been stimulated by purified RhD protein (▪). The effects on the inhibition of proliferation are shown when replicate cultures were treated with either (A) neutralizing anti–IL-10 antibody (■), or (B) blocking anti–CTLA-4 F(ab′)2 0.5 μg/mL (▨). The anti–CTLA-4 F(ab′)2 also inhibits IL-10 production by the PBMCs responding to the RhD peptides alone (●) compared with unstimulated cultures (○; C). In each panel, the mean of triplicate results from a representative experiment are illustrated (n = 4, from 2 patients).
