Western blot analysis of C/EBPα and C/EBPε expression during myeloid differentiation.

Left panel: Western blot analysis was performed using whole cell extracts prepared from uninduced (0) and ATRA-induced (24 h) MPRO cells. The blots were probed with C/EBPα and C/EBPε antibodies. Right panel: Western blot analyses were performed on whole cell extracts prepared from uninduced (0) or Epo-induced (4 d) 32Dwt18 cells. The blots were probed with C/EBPα and C/EBPε antibodies as outlined in “Materials and methods.” The molecular weights of the proteins are indicated in kilodaltons.