![Fig. 2. CML-DCs are defective in antigen processing. / DCs were generated from healthy individuals (○) and from CML patients (●): patient 1 (A,D), patient 2 (B,E), and patient 7 (C,F). Immature HLA-DR7–expressing DCs were pulsed overnight with either whole TT (0.008 IU/mL) (A-C) and HA307-319 peptide (10 μg/mL) (D-E) or TT947-967 peptide (10 μg/mL) (F). Different numbers of irradiated and prepulsed DCs were cultured in the presence of either TT-specific T-cell clones ([A] clone TT1; [B] clone TT1, solid lines, and clone CG1, dotted lines; [C] clone JNI27) or HA307-319–specific T-cell clone ([D-E] clone 7P61) or TT947-967–specific T-cell clone ([F] clone JNI27). After 2 days, 3HTdR was added and thymidine incorporation was measured after 20 hours. The data are expressed as counts per minute (cpm) ± standard deviations (SD). Results are representative of 15 experiments obtained with DCs derived from different patients as indicated in Table 1.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/9/10.1182_blood-2002-06-1841/4/h80934217002.jpeg?Expires=1724259678&Signature=HCN5R3wMbG6lB9mD10Yza4aKtDmjdjA94wDdzoP0RT7V7u2BFAHMeTs7SWhPALzXZq3qy~Y7eDBmCe0~p~h2ptCFHgtAb4KhCIBu7Swz7ucxXTeqcpXhz8QDN9HAkgElHAG8tPj9NvLDXaP87IpEnkl6TInS6KkZAXelZP0Kqxa~UGKWJ~mW0oGy~SZRlztmhlhr7n2p00rdOBzzxxBlyIxlaML0rY~w6dqvFvi3~Tw7DlyKjJoSbkmYzy2EvoDZ-1YbHy3StfGbXAPEk0p00NzlAa-P-D~0YgMJiAi2EklKyy~LGW4ydXv7dAxAUgaTcYLEUCFMc78ONB3HaFc1UA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CML-DCs are defective in antigen processing.
DCs were generated from healthy individuals (○) and from CML patients (●): patient 1 (A,D), patient 2 (B,E), and patient 7 (C,F). Immature HLA-DR7–expressing DCs were pulsed overnight with either whole TT (0.008 IU/mL) (A-C) and HA307-319 peptide (10 μg/mL) (D-E) or TT947-967 peptide (10 μg/mL) (F). Different numbers of irradiated and prepulsed DCs were cultured in the presence of either TT-specific T-cell clones ([A] clone TT1; [B] clone TT1, solid lines, and clone CG1, dotted lines; [C] clone JNI27) or HA307-319–specific T-cell clone ([D-E] clone 7P61) or TT947-967–specific T-cell clone ([F] clone JNI27). After 2 days, 3HTdR was added and thymidine incorporation was measured after 20 hours. The data are expressed as counts per minute (cpm) ± standard deviations (SD). Results are representative of 15 experiments obtained with DCs derived from different patients as indicated in Table 1.
