Western blots of chimeric antibodies.

Antibodies were separated by SDS-PAGE with gradient gels (4%-15%) under nonreducing (A) or reducing (B) conditions. After transfer onto nitrocellulose, immunoblots were incubated with peroxidase-labeled polyclonal goat antihuman κ–light chain antibody and goat antihuman IgG or rabbit antihuman IgA, respectively. Blots were developed with enhanced chemoluminescent reaction reagent (ECL). Thus, the correct size of all isotypes of chimeric HLA class II antibody F3.3 was demonstrated.