Sandwich ELISA for antibody quantification.

ELISA plates were coated with polyclonal antibodies against human IgA or human IgG. After chimeric antibodies were added in serial dilutions, peroxidase-labeled antihuman κ antibody was used for detection. Polyclonal human IgA or rituxan served as standards for IgAs or IgGs, starting from 50 or 5 μg/mL, respectively. This assay also demonstrated the assembly of light and heavy chains of the chimeric antibodies. Data from 1 of 4 experiments with similar results are shown for IgG1, IgA1, and IgA2.