Phenotypic analysis of HUBEC-cultured cells engrafted in the bone marrow of NOD/SCID mice.

(A) Expression of human CD45⁺ cells within the bone marrow of a control NOD/SCID mouse that did not receive a transplant (top); expression of human CD45⁺ cells within the bone marrow of a NOD/SCID mouse that received a transplant of 1 × 10⁶fresh ABM CD34⁺ cells (middle); and expression of human CD45⁺ cells within the bone marrow of a NOD/SCID mouse that received a transplant of the progeny of 1 × 10⁶ ABM CD34⁺ following coculture with HUBECs (bottom). Isotype controls are shown at left. (B) Lineage distribution of engrafted human cells within a representative mouse that received a transplant of HUBEC-cultured cells. Panel Bi, murine marrow stained with isotype control IgG1-FITC and IgG1–peridinin chlorophyll A protein (IgG1-PerCP). Panel Bii, staining of murine marrow with anti-human CD45-PerCP and anti-murine CD45-FITC, showing both human and murine populations. Panel Biii, isotype staining with IgG1-FITC and IgG1-PE. Panel Biv, expression of human CD34-PE and CD38-FITC on engrafted cells within murine marrow. Panel Bv, staining with anti-CD19 and anti-CD3, demonstrating CD19 expression on engrafted human cells. Panel Bvi, expression of human CD33 and human CD13 on engrafted cells within the marrow.