Expression of eGFP in cultured normal CD34⁺PBSCs under optimum conditions for ex vivo transduction with lentivector or oncoretrovirus vector.

Shown is analysis over 28 days of culture of the percentage of normal CD34⁺PBSC expressing eGFP following 3 daily 7-hour transductions using 3rd-generation SIN lentivector–eGFP (○) or MFGS–eGFP (♦) under conditions stimulating proliferation (5 cytokines). Cells were analyzed by flow cytometry, and data were expressed as the percentage of cells that show greater fluorescence than the 99^th percentile of nontransduced cells in similar cultures. Results are representative of 6 experiments.