Fig. 1.
Induction of platelet thrombi by S aureusand S pyogenes.
(A) Human blood (donor 1) containing PPACK and mepacrine was perfused at 37°C over glass coverslips coated with the S aureusstrain Newman or the S pyogenes strain M5 Manfredo (coating suspension: 108 bacteria/mL). The 2 images, obtained by epifluorescence videomicroscopy, show platelets and platelet thrombi on an area of 45 000 μm2 after 8 minutes of perfusion at 400 s−1. (B) The corresponding surface coverage was measured after 7.5 to 8.5 minutes of perfusion at the indicated wall shear rates. The data are representative of 4 experiments with blood from different donors.
