Survival analysis of mice that underwent transplantation with HSCs and HSC+CMP/GMP against A fumigatus when administered at different times transplantation.

Following total body irradiation (9.5 Gy [950 cGy]), groups of mice were given 200 HSCs only (gray filled circles) or 200 HSCs plus 1 × 10⁴ CMP and 2 × 10⁴ GMP (black filled squares). All groups were infected with Aspergillus fumigatus (100 conidia, intravenously) at a designated time after transplantation (indicated by arrows). Animals not undergoing transplantation that were not infected served as controls for the lethal irradiation dose (broken line). (A) None of the groups survived when infected on day 3 after transplantation (D+3). All mice died within 3 to 5 days after infection. (B) All mice that received only HSCs succumbed to infection when challenged at D+7. However, the survival rate of the mice that underwent cotransplantation with CMP/GMP increased to 67% (P = .002) when similarly challenged. When the time of infection was delayed further to D+9 and D+11 (C,D, respectively), the survival rate of the CMP/GMP group improved to 100%, and mice with HSC-only transplantations showed improved survival rates (22% and 50%, respectively).