eGFP expression in secondary lymphoid organs from RAG-2 KO mice reconstituted with HSCs transduced with lentiviral vectors.

(A) Representative results of eGFP expression in CD4⁺ (left panels), CD8⁺ (middle panels), and B220⁺ cells (right panels) in lymphoid organs of reconstituted RAG-2 KO mice with HSCs transduced with the nonspecific LvPGK (upper panels), the T-specific LvCD4 (middle panels), or the CD4-specific LvCD4-Sil (lower panels) lentiviral vectors. Numbers indicate percentages of eGFP⁺ cells; MFI indicates mean fluorescence intensity of eGFP⁺ cells. (B) Specificity index in various cell subsets after reconstitution of RAG-2 KO mice. The histograms shown represent the index of specificity relative to the CD4 subset in CD8⁺, B220⁺, NK1.1⁺, and CD4^loCD11c⁺ cells and was calculated as the ratio between the frequency of eGFP^hi cells in the subset of interest divided by the frequency of eGFP^hi in the CD4 subset for each vector. A lower index relative to the CD4 subset, therefore, indicates better specificity. Results are the average ± SD of the index obtained from 3 mice except B cells with the LvCD4 vector and NK cells with the LvCD4-Sil, where results were calculated from 2 mice. When applicable, Studentt test was used to calculate a P value relative to the CD4 subset, as indicated by the asterisk (0.01  < P ≤ .05) and (P ≤ .01) above each histogram.