Fig. 1.
Fig. 1. Schematic representation of the gene transfer vectors. / Lentiviral vectors LvCD4 (A) and LvCD4-Sil (B). SD indicates splice donor; SA, splice acceptor; ψ, packaging signal; GA-RRE, truncated gag sequence with therev-responsive element; cppT (central polypurine tract of HIV); CD4pmE, human CD4 minimal promoter/murine enhancer cassette (590 bp); Sil, human CD4 silencer (484 bp); Wpre, posttranscriptionalcis-acting regulatory element of the woodchuck hepatitis virus (587 bp); LTR-SIN, self-inactivating 3′ LTR (deleted of 400 bp in the U3 region). Enzymatic sites used for cloning the minimal promoter are indicated together with the eGFP and the Wpre (ClaI/Asp718).

Schematic representation of the gene transfer vectors.

Lentiviral vectors LvCD4 (A) and LvCD4-Sil (B). SD indicates splice donor; SA, splice acceptor; ψ, packaging signal; GA-RRE, truncated gag sequence with therev-responsive element; cppT (central polypurine tract of HIV); CD4pmE, human CD4 minimal promoter/murine enhancer cassette (590 bp); Sil, human CD4 silencer (484 bp); Wpre, posttranscriptionalcis-acting regulatory element of the woodchuck hepatitis virus (587 bp); LTR-SIN, self-inactivating 3′ LTR (deleted of 400 bp in the U3 region). Enzymatic sites used for cloning the minimal promoter are indicated together with the eGFP and the Wpre (ClaI/Asp718).

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