Proliferation of cells from patients with trisomy 8 and monosomy 7 in the presence of Fas agonist or Fas antagonist.

BMMNCs were cultured with and without Fas agonist monoclonal antibody mAb (CH11) or Fas antagonist mAb (ZB4). Slides were prepared, and FISH was performed with labeled centromeric probes for chromosomes 8 and 7 prior to culture and on days 4, 7, and 14. Days 0 and 4 are shown above (no change in expression was seen between days 4 and 14). The proportion of cells with trisomy 8 is seen in panel A; those with 5q− and monosomy 7 are seen in panel B. *Samples from patients with a history of aplastic anemia receiving CsA.