Comparison of CD95 expression on cells with trisomy 8 and monosomy 7 and normal cells from the same donor.

BM was obtained from 2 patients with trisomy 8 and 2 patients with monosomy 7 and stained with CD3-PE and CD95-FITC mAbs. CD3⁻ cells were positively selected and sorted into CD95⁻ and CD95⁺ aliquots, which were prepared for FISH with fluorescent probes for chromosome 7 or 8. The proportion of cells with each cytogenetic abnormality is shown in panel A. An example of stained cells in the Fas⁻ and positive aliquots are seen in panel B (trisomy 8; chromosome 8 is orange; chromosome 7, a control, is green) and panel C (monosomy 7; chromosome 7). Trisomy 8 sample on the left was obtained from a patient with a history of aplastic anemia.