Fig. 6.
Effect of 2ME2 on genes encoding Hsps and ubiquitin in Dex-sensitive (MM.1S) and Dex-resistant (MM.1R) MM cells.
MM.1R cells were treated with 3 μM 2ME2 for the indicated times. (A-C) Total cell lysates were separated by 12.5% SDS-PAGE and analyzed by immunoblotting (IB) with anti-Hsp70, anti-Hsp90, or anti-Hsp27 Abs (upper panels) or anti-SHP2 (lower panels) Abs. (D) Total cellular RNA from MM.1S, MM.1R, and 2ME2-treated MM.1R cells was hybridized to 32P-labeled ubiquitin (UBC) and GAPDH cDNA probes.
