Effect of 2ME2 on cytoskeleton and cell cycle regulatory proteins.

MM.1S cells were treated with 3 μM 2ME2 for the indicated times. (A-B) Total cell lysates were separated by 12.5% SDS-PAGE and analyzed by immunoblotting (IB) with antitubulin or antiactin Abs (upper panels) or anti-SHP2 (lower panels) Abs. (C) 2ME2 affects VEGF-induced migration of MM.1S cells. MM.1S MM cells were treated with rVEGF (10 ng) for 24 hours, followed by exposure to 3 μM 2ME2 and analysis in a transwell migration assay. (D-F) Total cell lysates were separated by 12.5% SDS-PAGE and analyzed by immunoblotting (IB) with anti–cyclin B or anti–c-myc, or anti-p27 Abs (upper panels) or anti-SHP2 (lower panels) Abs. FL indicates full length; CF, cleaved fragment.