Fig. 5.
![Fig. 5. IL-21 inhibits IL-4–induced germ line Cε transcription in B cells. / (A) Purified splenic B cells were stimulated with LPS (10 μg/mL) in the presence of IL-4 (20 ng/mL) or IL-21 (10 ng/mL) or both for 16 hours. Total cellular RNA was prepared from these cells and RT-PCR for germ line Cε, Cγ1, and Cγ2a transcripts as well as β-actin (as a control) was performed. Shown are representative data from 5 independent experiments. (B) Purified splenic B cells were stimulated with LPS (1 μg/mL) in the presence of IL-4 (20 ng/mL) or IL-21 (10 ng/mL) or both for 48 hours with [3H]-thymidine added for the final 12 hours. Data are mean ± SD from 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/13/10.1182_blood-2002-04-1115/4/h82423553005.jpeg?Expires=1726834568&Signature=XWn2JtdwrRuVhh3Wl5KzAn13Yp1RazU8eAnDmHyqZMOH9Z4ACjXyn-HwovGv7yQrDHsQQZSyp1onO-Le9SY99Q7FJ8Mguipdc0cEhprxukY5jxPvbVLcQFWOROaHQrYVaRoWLxMxE5jJNyKQs3wLF~4K6I2mIZNhy-CbmWGxHH162GPPC-YhhHRwKhrJAXAXnfIT7u5rZyhNvctJUVGQ0VaxbL--HL6L5mQ58cRXD3~uGzJ3t-cRE2uca5XX7ng0Va5Gt-A0i5SDOj7M7kgBBdCyXasL0HGixRn6FDpzuinuWCMJLz9cC3WPbiDFL7NI4nTOOPGgPFzUapcebkT~rg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-21 inhibits IL-4–induced germ line Cε transcription in B cells.
(A) Purified splenic B cells were stimulated with LPS (10 μg/mL) in the presence of IL-4 (20 ng/mL) or IL-21 (10 ng/mL) or both for 16 hours. Total cellular RNA was prepared from these cells and RT-PCR for germ line Cε, Cγ1, and Cγ2a transcripts as well as β-actin (as a control) was performed. Shown are representative data from 5 independent experiments. (B) Purified splenic B cells were stimulated with LPS (1 μg/mL) in the presence of IL-4 (20 ng/mL) or IL-21 (10 ng/mL) or both for 48 hours with [3H]-thymidine added for the final 12 hours. Data are mean ± SD from 3 independent experiments.
