Inhibition of contact- and soluble factor–dependent MM cell-induced osteoclastogenesis by antibodies against MIP-1α and MIP-1β or CCR5.

Rabbit bone marrow cells were cocultured with MM cells or cultured with MM cell-conditioned media (CM). CM were added at a final dilution of 1:2. Anti–MIP-1α and anti–MIP-1β neutralizing antibodies were used in combination at 20 μg/mL for each. Goat IgG was used as a control. Results were shown as a percent increase in the number of TRAP⁺ MNCs from the control culture with rabbit bone marrow cells alone and expressed as a mean of 5 samples with an error bar of SD. * indicates significantly different from the control IgG-treated culture in the number of TRAP⁺ MNCs (P < .05, ANOVA with Scheffe post hoc tests).