TSA and NaB affect NF-κB and p38 MAPK signaling pathways in RAW-D cells

. (A) TSA or NaB suppresses the nuclear translocation of NF-κB p65 in RAW-D cells stimulated with TNF-α. RAW-D cells were treated without or with TSA (100 nM) or NaB (1 mM) for 24 hours, followed by treatment with TNF-α for 30 minutes. Nuclear extracts were prepared and analyzed by Western blotting using NF-κB p65 antibody. (B) TSA or NaB suppresses activation of p38 MAPK in RAW-D cells stimulated with sRANKL. RAW-D cells were preincubated with or without TSA (100 nM) or NaB (1 mM) for 24 hours and stimulated with sRANKL (100 ng/mL) for 30 minutes. Whole-cell lysates were prepared and analyzed by Western blotting using phosphorylated p38 MAPK antibody. The bottom lane indicates equal loading of protein probed for actin antibody.