TSA or NaB inhibits osteoclast differentiation of RAW-D cells stimulated with sRANKL.
Demonstration of TRAP+ cells in RAW-D cells stimulated with sRANKL in the presence (B) or absence (A) of TSA. (C) Dose-dependent effect of TSA or NaB on the formation of TRAP+ MNCs in RAW-D stimulated with sRANKL. RAW-D cells (1.5 × 105 cells/mL) were cultured for 3 days in the presence of sRANKL (30 ng/mL) with various concentrations of TSA or NaB. TSA or NaB was added after 24 hours of incubation. Cells were then fixed and stained for TRAP. The number of TRAP+ MNCs was counted in each well. Each value represents the mean ± SEM of quadruplicate cultures. Data were analyzed by Student t test. *P < .001 compared with the culture without TSA. Scale bars = 100 μm.
