TSA does not affect the formation of M-CSF–induced NSE⁺ cells.

Demonstration of NSE⁺ cells in rat bone marrow culture stimulated with M-CSF in the presence (B) or absence (A) of TSA. Scale bars = 100 μm. (C) Dose effect of TSA on the formation of NSE⁺ cells in stroma-free rat bone marrow culture. Stroma-free bone marrow cells were cultured in the presence of hM-CSF (1 ng/mL) for 4 days. Various concentrations of TSA were added after 24 hours of incubation. The cells were then fixed and stained for NSE (A-B), and the number of NSE⁺ cells was counted in each well (C). Each bar represents the mean ± SEM of quadruplicate cultures.