Demonstration of TRAP⁺ MNCs in the mouse bone marrow culture treated with sRANKL and M-CSF in the presence of TSA or NaB.

Mouse bone marrow cells were cultured in the presence of hM-CSF (50 ng/mL) and sRANKL (50 ng/mL) with or without TSA or NaB for 4 days. (A) Without TSA or NaB; (B) with 20 nM TSA; (C) with 1 mM NaB. The cells were then fixed and stained for TRAP. A number of TRAP⁺ MNCs were observed in the culture without TSA or NaB (A). No TRAP⁺ MNCs were observed in cultures with TSA or NaB (B-C). Scale bars = 100 μm.