Assessment of CCL19-induced dendritic extension in SPDCs.

The SPDCs were generated by culturing splenocytes with GM-CSF and fibroblast supernatant for 14 days. SPDCs were activated by LPS and used as mature SPDCs. The mature SPDCs were incubated with CCL19 (20 ng/mL) or CCL21 (100 ng/mL) for 90 minutes. The morphologic changes were analyzed by invert microscopy (original magnification × 30). The results are representative of 4 independent experiments.